The NAD synthetase NadE (OutB) of Bacillus subtilis is a σB-dependent general stress protein

Contributions of individual σB-dependent general stress genes to oxidative stress resistance of Bacillus subtilis.

The general stress regulon of Bacillus subtilis comprises approximately 200 genes and is under the control of the alternative sigma factor σ(B). The activation of σ(B) occurs in response to multiple physical stress stimuli as well as energy starvation conditions. The expression of the general stress proteins provides growing and stationary nonsporulating vegetative cells with nonspecific and br...

Salmonella typhimurium nit is nadE: defective nitrogen utilization and ammonia-dependent NAD synthetase.

S. typhimurium nit mutants are defective in nitrogen assimilation, despite having normal levels of assimilatory enzymes. Complementation, enzyme assays, and genetic mapping show that nit is nadE. We present evidence that ammonia, not glutamine, is the physiological substrate for eubacterial NAD synthetases and that low activity completely accounts for the mutant phenotype.

The σB-Dependent Promoter of the Bacillus subtilis sigB Operon Is Induced by Heat Shock

The general stress protein Ctc of Bacillus subtilis is a ribosomal protein.

Cells respond to stress conditions by synthesizing general or specific stress proteins. The Ctc protein of Bacillus subtilis belongs to the general stress proteins. The synthesis of Ctc is controlled by an alternative sigma factor of RNA polymerase, sigmaB. Sequence analyses revealed that Ctc is composed of two domains, an N-terminal domain similar to the ribosomal protein L25 of Escherichia co...

Expression of the sigmaB-dependent general stress regulon confers multiple stress resistance in Bacillus subtilis.

The alternative sigma factor sigmaB of Bacillus subtilis is required for the induction of approximately 100 genes after the imposition of a whole range of stresses and energy limitation. In this study, we investigated the impact of a null mutation in sigB on the stress and starvation survival of B. subtilis. sigB mutants which failed to induce the regulon following stress displayed an at least ...